Joint BioEnergy Institute 3 articles published in JoVE Environment Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions Jian Gao1,2, Joelle Sasse1,2, Kyle M. Lewald1,2, Kateryna Zhalnina1,2, Lloyd T. Cornmesser1,2, Todd A. Duncombe3, Yasuo Yoshikuni2, John P. Vogel2, Mary K. Firestone4, Trent R. Northen1,2 1Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 2Joint Genome Institute, Department of Energy, 3Joint BioEnergy Institute, 4Department of Environmental Science Policy and Management, University of California This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory conditions. Biochemistry Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE Venkataramana R. Pidatala*1,2, Amir Mahboubi*1,2, Jenny C. Mortimer1,2 1Joint BioEnergy Institute, 2Environmental and Systems Biology, BioSciences Division, Lawrence Berkeley National Laboratory A protocol for the structural analysis of polysaccharides by gel electrophoresis (PACE), using mannan as an example, is described. Biology Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements Prajakta Pradhan Mitra1,2, Dominique Loqué1,2 1Feedstocks Division, Joint Bioenergy Institute, 2Physical Biosciences Division, Lawrence Berkeley National Laboratory Plant cell wall composition varies between tissue types and can include lignin, cellulose, hemicelluloses, and pectin. Various staining techniques have been developed to visualize differences at the cell-type level. This paper is a compilation of commonly used cell wall staining techniques.
