USDA-ARS, Crop Improvement and Protection Unit 1 article published in JoVE Environment Procedures of Laboratory Fumigation for Pest Control with Nitric Oxide Gas Yong-Biao Liu1, Xiangbing Yang2, Tiffany Masuda1 1Crop Improvement and Protection Unit, USDA-ARS, 2The University of California Davis This paper describes nitric oxide (NO) fumigation protocols for postharvest pest control. Fumigation chambers are flushed with nitrogen (N2) to establish ultralow oxygen conditions before NO is injected. At the end, chambers are flushed with N2 to dilute NO before exposing products to ambient air to prevent exposure to NO2.