BioMarin Pharmaceutical Inc 1 article published in JoVE Immunology and Infection Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay Ruby Cheung1, Gregory W. deHart1, Lynne Jesaitis1, Stephen J. Zoog1, Andrew C. Melton1 1BioMarin Pharmaceutical Inc Here we present a cell-based flow cytometry method to detect neutralizing antibodies or other factors that interfere with the cellular uptake of enzyme replacement therapies in a human matrix, such as cerebral spinal fluid (CSF) or human serum.
