Parkinson’s Institute and Clinical Center 2 articles published in JoVE Neuroscience Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors Kelsey Lee*1, Ou Chong Saetern*1, Andrew Nguyen1, Leonardo Rodriguez1, Birgitt Schüle1 1Parkinson’s Institute and Clinical Center The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells. Medicine Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts Malini Vangipuram1, Dennis Ting1, Sam Kim1, Robert Diaz1, Birgitt Schüle1 1Basic Research Department, The Parkinson's Institute The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.
