Janssen Parmaceutical Companies of Johnson and Johnson 1 article published in JoVE Immunology and Infection Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations Stuart T. Perry1, Elissa Keogh1, Mike Morton1, Wouter Koudstaal2, Gabriel Pascual1 1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.
