Thermo Fisher Scientific 2 articles published in JoVE Engineering Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve Yifei Liu1, Yong-Ung Lee2, Tai Yi2, Ken Wu3, Cedric Bouchet-Marquis3, Han Chan3, Christopher K. Breuer2, David W. McComb1 1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, 2 Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures. Immunology and Infection Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR Suman K. Pradhan1, Jerry Guzman1, Carl Dargitz1, Stephanie Switalski2, Mark Landon1, Sven Olek3, Bjoern Samans3, Ulrich Hoffmueler3, Uma Lakshmipathy1 1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
