UC San Diego 1 article published in JoVE Chemistry Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy Jackson C. Wagner1, Bin Yang1,2, Zishan Wu1, Wei Xiong1,3,4 1Department of Chemistry and Biochemistry, UC San Diego, 2State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 3Materials Science and Engineering Program, UC San Diego, 4Department of Electrical and Computer Engineering, UC San Diego A multimodal, rapid hyperspectral imaging framework was developed to obtain broadband vibrational sum-frequency generation (VSFG) images, along with brightfield, second harmonic generation (SHG) imaging modalities. Due to the infrared frequency being resonant with molecular vibrations, microscopic structural and mesoscopic morphology knowledge is revealed of symmetry-allowed samples.