University of Connecticut Health Center 9 articles published in JoVE Biology A Finite Element Approach for Locating the Center of Resistance of Maxillary Teeth Bill Luu1, Edward Anthony Cronauer2, Vaibhav Gandhi1, Jonathan Kaplan3, David M. Pierce3,4, Madhur Upadhyay1 1Division of Orthodontics, University of Connecticut Health, 2Private Practice, Miami, FL, 3Department of Biomedical Engineering, University of Connecticut, 4Department of Mechanical Engineering, University of Connecticut This study outlines the necessary tools for utilizing low-dose three-dimensional cone beam-based patient images of the maxilla and maxillary teeth to obtain finite element models. These patient models are then used to accurately locate the CRES of all the maxillary teeth. Developmental Biology Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution Leighton H. Duncan1,5, Mark W. Moyle1,5, Lin Shao1,5, Titas Sengupta1,5, Richard Ikegami1,5, Abhishek Kumar4,5, Min Guo4,5, Ryan Christensen4,5, Anthony Santella2,5, Zhirong Bao2,5, Hari Shroff4,5, William Mohler3,5, Daniel A. Colón-Ramos1,5,6 1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment. Bioengineering Force System with Vertical V-Bends: A 3D In Vitro Assessment of Elastic and Rigid Rectangular Archwires Madhur Upadhyay1, Raja Shah2, Sachin Agarwal3, Meenakshi Vishwanath4, Po-Jung Chen5, Takafumi Asaki6, Donald Peterson7 1Division of Orthodontics, University of Connecticut Health, 2Private Practice, 3Department of Orthodontics, University of Melbourne, 4Department of Orthodontics, University of Nebraska Medical Center, 5Department of Craniofacial Sciences, University of Connecticut Health, 6Biomedical Engineering, University of Hartford, 7Department of Mechanical Engineering, College of Engineering and Engineering Technology, Northern Illinois University The method presented here is designed to construct and validate an in vitro 3D model capable of measuring the force system generated by different archwires with V-bends placed between two brackets. Additional objectives are to compare this force system with different types of archwires and to previous models. Biology A Morphometric and Cellular Analysis Method for the Murine Mandibular Condyle Eliane H. Dutra1, Mara H. O'Brien1, Alexandro Lima1, Ravindra Nanda1, Sumit Yadav1 1Division of Orthodontics, University of Connecticut Health Center This manuscript presents methods for analyzing morphometric and cellular changes within the mandibular condyle of rodents. Biology High-Throughput, Multi-Image Cryohistology of Mineralized Tissues Nathaniel A. Dyment1, Xi Jiang1, Li Chen1, Seung-Hyun Hong2, Douglas J. Adams3, Cheryl Ackert-Bicknell4, Dong-Guk Shin2, David W. Rowe1 1Department of Reconstructive Sciences, University of Connecticut Health Center, 2Department of Computer Science and Engineering, University of Connecticut, 3Department of Orthopaedic Surgery, University of Connecticut Health Center, 4Department of Orthopaedics, University of Rochester In this manuscript, we present a high-throughput, semi-automated cryohistology platform to produce aligned composite images of multiple response measures from several rounds of fluorescent imaging on frozen sections of mineralized tissues. Neuroscience 3D Modeling of the Lateral Ventricles and Histological Characterization of Periventricular Tissue in Humans and Mouse Rebecca L. Acabchuk1, Ye Sun1, Richard Wolferz, Jr.1, Matthew B. Eastman1, Jessica B. Lennington1, Brett A. Shook1, Qian Wu2, Joanne C. Conover1 1Department of Physiology and Neurobiology, University of Connecticut, 2Department of Anatomic Pathology and Laboratory Medicine, University of Connecticut Health Center Using MRI scans (human), 3D imaging software, and immunohistological analysis, we document changes to the brain’s lateral ventricles. Longitudinal 3D mapping of lateral ventricle volume changes and characterization of periventricular cellular changes that occur in the human brain due to aging or disease are then modeled in mice. Neuroscience An In Vitro Model for the Study of Cellular Pathophysiology in Globoid Cell Leukodystrophy Kumiko I. Claycomb1, Kasey M. Johnson1, Ernesto R. Bongarzone2, Stephen J. Crocker1 1Department of Neuroscience, University of Connecticut Health Center, 2Department of Anatomy and Cell Biology, University of Illinois at Chicago Globoid cells are a defining pathological feature of Krabbe disease, a leukodystrophy currently lacking an effective long-term therapy. We have developed a cell culture model to study the innate biology and pathogenic potential of activated microglia and their transformation into globoid cells. Bioengineering The Portable Chemical Sterilizer (PCS), D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military Christopher J. Doona1, Florence E. Feeherry1, Peter Setlow2, Alexander J. Malkin3, Terrence J. Leighton4 1United States Army-Natick Soldier RD&E Center, Warfighter Directorate, 2Department of Molecular Biology and Biophysics, University of Connecticut Health Center, 3Lawrence Livermore National Laboratory, 4 The Portable Chemical Sterilizer (PCS) is a revolutionary, energy-independent, almost waterless sterilization technology for Army medical units. The PCS generates chlorine dioxide from dry reagents mixed with water on-site, at-will, and at point-of-use (PoU) in a plastic suitcase. The Disinfectant-sprayer for Foods and ENvironmentally-friendly Sanitation (D-FENS) and the Disinfectant for ENvironmentally-friendly Decontamination, All-purpose (D-FEND ALL) produce aqueous chlorine dioxide in a collapsible spray bottle and other potential embodiments. These versatile decontamination technologies kill microbes in myriad diverse Dual-use applications for military and civilian consumers. Neuroscience Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster Jared K. Woods1, Suzanne Kowalski1, Blanka Rogina1 1Genetics and Developmental Biology, School of Medicine, University of Connecticut Health Center Drosophila melanogaster are useful in studying genetic or environmental manipulations that affect behaviors such as spontaneous locomotor activity. Here we describe a protocol that utilizes monitors with infrared beams and data analysis software to quantify spontaneous locomotor activity.