Yerkes National Primate Research Center 1 article published in JoVE Immunology and Infection Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals Kateena Addae Konadu1, Ming Bo Huang1, William Roth1, Wendy Armstrong2, Michael Powell1, Francois Villinger3,4, Vincent Bond1 1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.