Center for Food Safety 1 article published in JoVE Biology Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples Ji-Yeon Hyeon1, David A. Mann1, Anna M. Townsend1, Xiangyu Deng1 1Center for Food Safety Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples.
