Lake Forest College 1 article published in JoVE Genetics Quantifying Abdominal Pigmentation in Drosophila melanogaster Omid Saleh Ziabari1, Alexander W. Shingleton1 1Department of Biology, Lake Forest College This work presents a method to quickly and precisely quantify the abdominal pigmentation of Drosophila melanogaster using digital image analysis. This method streamlines the procedures between phenotype acquisition and data analysis and includes specimen mounting, image acquisition, pixel value extraction, and trait measurement.