Ann and Robert H. Lurie Children's Hospital of Chicago 1 article published in JoVE Immunology and Infection A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis Guillermo J. Ares1, Christie Buonpane1, Carrie Yuan1, Douglas Wood1, Catherine J. Hunter1,2 1Department of Pediatrics, Northwestern University, 2 Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.
