Tymora Analytical Operations 1 article published in JoVE Biochemistry Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis Yi-Kai Liu1, Zhuojun Luo1, Anton Iliuk1,2, Weiguo Andy Tao1,2,3,4 1Department of Biochemistry, Purdue University, 2Tymora Analytical Operations, 3Department of Chemistry, Purdue University, 4Purdue Institute for Cancer Research, Purdue University The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and phosphoproteomics.
