National Institute on Aging, NIH 5 articles published in JoVE Biology Techniques to Induce and Quantify Cellular Senescence Nicole Noren Hooten1, Michele K. Evans1 1Laboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence. Medicine Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle Vidhya Kumar1, Henry Chang1, David A. Reiter2, David P. Bradley3, Martha Belury4, Shana E. McCormack5, Subha V. Raman1 1Davis Heart and Lung Research Institute, The Ohio State University, 2Laboratory of Clinical Investigation, National Institute on Aging, 3Division of Endocrinology, Diabetes and Metabolism, The Ohio State University, 4Department of Human Sciences, Human Nutrition, The Ohio State University, 5Division of Endocrinology and Diabetes, Department of Pediatrics, University of Pennsylvania This work demonstrates the feasibility of an in vivo phosphorus-31 magnetic resonance spectroscopy (31PMRS) technique to quantify mitochondrial oxidative phosphorylation (OXPHOS) capacity in human skeletal muscle. Medicine A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics Natalia Petrasheskaya1, Hyun-Jin Tae1,2, Ismayil Ahmet1, Mark I. Talan1, Edward G. Lakatta1, Li Lin1 1Laboratory of Cardiovascular Sciences, National Institute on Aging, 2Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University The rat carotid balloon injury model described below allows researchers to evaluate drugs or therapeutics that negate injury-induced arterial hyperplasia. Detailed pre-surgical preparation, surgical procedure, and post-surgical cares of the animal are described. Immunology and Infection Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network Nicolas Dutzan*1, Loreto Abusleme*1, Joanne E. Konkel2,3, Niki M. Moutsopoulos1 1Oral Immunity and Inflammation Unit, NIDCR, NIH, 2Manchester Immunology Group, Faculty of Life Sciences, University of Manchester, 3Manchester Collaborative Centre for Inflammation Research, University of Manchester We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva. Medicine Pulse Wave Velocity Testing in the Baltimore Longitudinal Study of Aging Melissa David1, Omar Malti1, Majd AlGhatrif1, Jeanette Wright1, Marco Canepa1, James B. Strait1 1Clinical Research Branch, National Institute of Aging Pulse wave velocity (PWV) measurement assesses arterial stiffness by a tonometry-based system that measures the speed with which arterial pressure wave travels along the arterial tree, typically approximating the time that it takes this wave to travel from the descending aorta (using the carotid artery as a surrogate) to the femoral artery. Carotid-femoral PWV increases two- to threefold across the adult lifespan.
