University of Maryland School of Pharmacy 2 articles published in JoVE Medicine Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport Jessica Felton1, Kunrong Cheng2, Anan Said2, Aaron C. Shang2, Su Xu3, Diana Vivian4, Melissa Metry5, James E. Polli5, Jean-Pierre Raufman2,6 1Department of Surgery, University of Maryland School of Medicine, 2Department of Medicine, University of Maryland School of Medicine, 3Department of Radiology, University of Maryland School of Medicine, 4Food and Drug Administration, 5Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 6VA Maryland Health Care System Tools to diagnose bile acid malabsorption and measure bile acid transport in vivo are limited. An innovative approach in live animals is described that utilizes combined proton (1H) plus fluorine (19F) magnetic resonance imaging; this novel methodology has translational potential to screen for bile acid malabsorption in clinical practice. Biology Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1 Karthika Natarajan1,2, Yi Xie1,3, Takeo Nakanishi4, Rebecca S. Moreci5,6, Pancharatnam Jeyasuria7, Arif Hussain1,3,8,9, Douglas D. Ross1,3,8,9,10,11 1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6 With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.
