University of Missouri-Kansas City 2 articles published in JoVE Biology Circadian Entrainment of Drosophila Melanogaster Austin O. Dada1, Minh Q. Nguyen1, Shea M. Peterson1, Vy T. Ngo1, Dayanne V. Cornelio-Parra1, Bwaar S. Omer1, Ada Thapa2, Sarah R. Rapp1, Veronica J. Cloud1, Ryan D. Mohan1 1School of Biological and Chemical Sciences, University of Missouri - Kansas City, 2School of Public Health, University of Maryland Here, we detail how to synchronize Drosophila to a circadian day. This is the first, and most important step necessary for studying biological rhythms and chronobiology. Biochemistry Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase Chayan Bhattacharya*1, Aninda Sundar Dey*1, Navid J. Ayon*1, William G. Gutheil1, Mridul Mukherji1 1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City Here, we present a protocol for an efficient single step purification of the active untagged human ten-eleven translocation-2 (TET2) 5-methylcytosine dioxygenase using ion-exchange chromatography and its assay using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach.
