University of New Mexico Health Science Center 1 article published in JoVE Immunology and Infection Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium Julie G. In*1, Jennifer Foulke-Abel*2, Elizabeth Clarke1, Olga Kovbasnjuk1 1Department of Internal Medicine, University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.
