University of New Mexico Health Sciences Center 3 articles published in JoVE Immunology and Infection A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs Ziming Cao1, Matthew J. Garcia2, Larry A. Sklar2,3,4,5, Angela Wandinger-Ness3,4, Zhichao Fan1 1Department of Immunology, School of Medicine, UConn Health, 2Center for Molecular Discovery, University of New Mexico Health Sciences Center, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center, 4Department of Pathology, University of New Mexico Health Sciences Center, 5Autophagy, Inflammation, & Metabolism (AIM) Center, University of New Mexico This protocol describes a flow cytometry-based, high-throughput screening method to identify small-molecule drugs that inhibit β2 integrin activation on human neutrophils. Behavior Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model Marena A. Montera1, Aleyah E. Goins1, Sascha R. A. Alles1, Karin N. Westlund1 1Department of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center Methods for simple, rapid induction of a back pain model in mice are provided here using an intraligament injection of urinary plasminogen activator. Biochemistry An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation Elizabeth M. Bailey1, Emanuel Salazar-Cavazos1, Rachel M. Grattan1, Michael J. Wester2, David J. Schodt2, Julian A. Rojo1, Keith A. Lidke2,3, Diane S. Lidke1,3 1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.
