Mount Sinai Hospital 2 articles published in JoVE Cancer Research An In Vivo Murine Sciatic Nerve Model of Perineural Invasion Sylvie Deborde*1, Yasong Yu*1, Andrea Marcadis1, Chun-Hao Chen1, Ning Fan2, Richard L. Bakst3, Richard J. Wong1 1Department of Surgery, Memorial Sloan Kettering Cancer Center, 2Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, 3Department of Radiation Oncology, Mount Sinai Hospital We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion. Developmental Biology The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System Enrica Bertin1, Martina Piccoli1, Chiara Franzin1, Andras Nagy2, Maria Mileikovsky2, Paolo De Coppi3, Michela Pozzobon1 1Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, 2Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 3Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.
