Institute for Systems Genetics 1 article published in JoVE Biology qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping Leslie A. Mitchell1, Nick A. Phillips1, Andrea Lafont2, James A. Martin1, Rupal Cutting2, Jef D. Boeke1 1Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, 2Roche Life Science, USA Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.
