Ohio State University Comprehensive Cancer Center and College of Medicine 1 article published in JoVE Biology Analysis of SCAP N-glycosylation and Trafficking in Human Cells Chunming Cheng1, Jeffrey Yunhua Guo1, Feng Geng1, Xiaoning Wu1, Xiang Cheng1, Qiyue Li1, Deliang Guo1 1Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center and College of Medicine We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP N-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.
