Cleveland Clinic 7 articles published in JoVE Bioengineering Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy Jihye Bae1, Jordan L. Clay2, Bhoj Raj Thapa1, David Powell3, Heidi Turpin3, Saghi Tasori Partovi3, Rachel Ward-Mitchell3, Balu Krishnan4, Andreas Koupparis5, Meriem Bensalem Owen2, Flavius D. Raslau2,6,7 1Department of Electrical and Computer Engineering, University of Kentucky, 2Department of Neurology, University of Kentucky, 3Department of Neuroscience, University of Kentucky, 4Cleveland Clinic, 5Cyprus Institute of Neurology and Genetics, 6Department of Radiology, University of Kentucky, 7Department of Neurosurgery, University of Kentucky This article details simultaneous electroencephalogram and functional magnetic resonance imaging (EEG-fMRI) recording procedures that can be used in both clinical and research settings. EEG processing procedures to remove imaging artifacts for clinical review are also included. This study focuses on the example of epilepsy during the interictal period. Cancer Research Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture Swetha J. Sundar1, Sajina Shakya2, Violette Recinos1, Christopher G. Hubert2 1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo. Engineering The Immersive Cleveland Clinic Virtual Reality Shopping Platform for the Assessment of Instrumental Activities of Daily Living Jay L. Alberts1,2, Morgan McGrath1,3, Mandy Miller Koop1, Colin Waltz1, Logan Scelina1, Kathryn Scelina1, Anson B. Rosenfeldt1 1Department of Biomedical Engineering, Cleveland Clinic, Lerner Research Institute, 2Neurological Institute, Center for Neurological Restoration, Cleveland Clinic, 3Case Western Reserve University, School of Medicine Virtual reality (VR) is a powerful yet underutilized approach to advance the diagnosis and treatment of neurological disease. The Cleveland Clinic Virtual Reality Shopping platform combines state-of-the-art VR content with an omnidirectional treadmill to quantify instrumental activities of daily living-a proposed prodromal marker of neurological disease. Biology Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria Dionysios C. Watson1,2,3, Sadie Johnson1, Akeem Santos1,4, Mei Yin5, Defne Bayik1, Justin D. Lathia1,3, Mohammed Dwidar1,3,4 1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health, Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research. Medicine A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice Elliott G. Richards*1, Jenna M. Rehmer*2, Melissa A. Mathes3, Emily L. Esakov1, Chad Braley1, Amy Joehlin-Price4, Andres Chiesa-Vottero4, Ofer Reizes1,5 1Cardiovascular and Metabolic Sciences, Lerner Research Institute; Women’s Health Institute, Cleveland Clinic, 2Women’s Health Institute, Cleveland Clinic, 3Department of Obstetrics and Gynecology, 983255 Nebraska Medical Center, University of Nebraska Medical Center, 4Anatomic Pathology, Cleveland Clinic, 5Gynecologic Oncology, Women’s Health Institute and Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Cleveland Clinic Many rodent models of endometriosis are limited by technical complexity, reproducibility, and/or need for immunocompromised animals or special reporter mice. We present a simplified system of lesion induction using any experimental mouse with an independently verifiable, objective scoring system and with no requirement for ovariectomy or survival surgery. Neuroscience Comprehensive Autopsy Program for Individuals with Multiple Sclerosis Ranjan Dutta*1, Kedar R. Mahajan*1,3, Kunio Nakamura*3, Daniel Ontaneda*2, Jacqueline Chen1, Christina Volsko1, Jessica Dudman1, Emilie Christie1, Jordon Dunham1, Robert J. Fox3, Bruce D. Trapp1 1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, 2Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 3Mellen Center for Treatment and Research in Multiple Sclerosis, Neurological Institute, Cleveland Clinic Multiple sclerosis is an inflammatory demyelinating disease with no cure. Analysis of brain tissue provides important clues to understanding the pathogenesis of the disease. Here we discuss the methodology and downstream analysis of MS brain tissue collected through a unique rapid autopsy program in operation at the Cleveland Clinic. Medicine Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments Jing Hao1, Anthony Magnelli2, Andrew Godley2, Jennifer S. Yu1,2 1Department of Cancer Biology, Cleveland Clinic, 2Department of Radiation Oncology, Cleveland Clinic Clinical linear accelerators can be used to determine biologic effects of a wide range of dose rates on cancer cells. We discuss how to set up a linear accelerator for cell-based assays and assays for cancer stem-like cells grown as tumorspheres in suspension and cell lines grown as adherent cultures.
