Corporal Michael J. Crescenz VA Medical Center 1 article published in JoVE Immunology and Infection Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions Clayton J. Otter1,2, Alejandra Fausto1,2, Li Hui Tan3,4, Susan R. Weiss1,2, Noam A. Cohen3,4 1Department of Microbiology, University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.
