The University of Tennessee Health Science Center (UTHSC) 1 article published in JoVE Biochemistry Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol Alexandria Slayden1, Kelsey North1, Shivantika Bisen1, Alex M. Dopico1, Anna N. Bukiya1, Avia Rosenhouse-Dantsker2 1Department of Pharmacology, Addiction Science and Toxicology, The University of Tennessee HSC, 2Department of Chemistry, University of Illinois at Chicago Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.
