James H. Quillen VA Medical Center 1 article published in JoVE Medicine Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography Stephanie L. C. Scofield1, Krishna Singh1,2 1Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, 2James H. Quillen Veterans Affairs Medical Center During murine myocardial ischemia/reperfusion surgery, correct placement of the occluding ligature is typically confirmed by visible observation of myocardial pallor. Herein, a method of electrocardiographically confirming ischemia and reperfusion, to supplement observed myocardial pallor, is demonstrated in male C57Bl/6 mice.