The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences 7 articles published in JoVE Biochemistry Detection of Protein S-Acylation using Acyl-Resin Assisted Capture Ritika Tewari*1, Savannah J. West*1,2, Bieerkehazi Shayahati1, Askar M. Akimzhanov1 1Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples. Cancer Research Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells Ruoji Zhou1,2, An Xu1, Jian Tu1,3, Mo Liu1, Julian A Gingold4, Ruiying Zhao1, Dung-Fang Lee1,2,5,6 1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts. Engineering Guidelines and Experience Using Imaging Biomarker Explorer (IBEX) for Radiomics Rachel B. Ger1,2, Carlos E. Cardenas1,2, Brian M. Anderson1,2, Jinzhong Yang1,2, Dennis S. Mackin1, Lifei Zhang1, Laurence E. Court1,2,3 1Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, 2UTHealth Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, 3Department of Imaging Physics, The University of Texas MD Anderson Cancer Center We describe IBEX, an open-source tool designed for medical imaging radiomics studies, and how to use this tool. In addition, some published works that have used IBEX for uncertainty analysis and model building are showcased. Neuroscience Novel Assay for Cold Nociception in Drosophila Larvae Heather N. Turner1,2,5, Christian Landry3, Michael J. Galko1,2,4 1Department of Genetics, UT MD Anderson Cancer Center, 2Neuroscience Program, Graduate School of Biomedical Sciences at Houston, 3ProDev Engineering, 4Genes and Development Program, Graduate School of Biomedical Sciences at Houston, 5Section of Neurobiology, University of Southern California Here we demonstrate a novel assay to study cold nociception in Drosophila larvae. This assay utilizes a custom-built Peltier probe capable of applying a focal noxious cold stimulus and results in quantifiable cold-specific behaviors. This technique will allow further cellular and molecular dissection of cold nociception. Developmental Biology Spatial and Temporal Analysis of Active ERK in the C. elegans Germline Amanda L. Gervaise1, Swathi Arur1,2 1Program in Developmental Biology, Baylor College of Medicine, 2Department of Genetics, UT MD Anderson Cancer Center We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available. Developmental Biology Technique to Target Microinjection to the Developing Xenopus Kidney Bridget D. DeLay1, Vanja Krneta-Stankic1,2, Rachel K. Miller1,2,3,4 1Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, 2Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics, University of Texas MD Anderson Cancer Center Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos. Immunology and Infection Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors Haiyan S. Li1, Stephanie S. Watowich1,2 1Department of Immunology, The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.
