Texas A&M Agrilife Center at Dallas 1 article published in JoVE Immunology and Infection Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes Jeanmarie Verchot1, Aastha Thapa2, Dulanjani Wijayasekara3, Peter R. Hoyt4 1Texas A&M Agrilife Center at Dallas, 2Noble Research Center, Oklahoma State University, 3Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, 4Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
