University of Utah School of Medicine 7 articles published in JoVE Medicine Three-Dimensional Cephalometric Landmark Annotation Demonstration on Human Cone Beam Computed Tomography Scans Konstantinia Almpani1,2, Anna Adjei2, Denise K. Liberton1, Payal Verma1, Man Hung*2,3,4, Janice S. Lee*1 1National Institute of Dental and Craniofacial Research, 2College of Dental Medicine, Roseman University of Health Sciences, 3University of Utah School of Medicine, 4George E. Wahlen VA Medical Center Presented here is a detailed protocol for the conduction of three-dimensional cephalometric analysis with the use of human cone beam computed tomography scans. Developmental Biology Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos Hyung-Seok Kim1, Jan L. Christian2 1Department of Neurobiology, University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies, University of Utah, School of Medicine When Transforming Growth Factor ß family precursor proteins are ectopically expressed in Xenopus laevis embryos, they dimerize, get cleaved and are secreted into the blastocoele, which begins at the late blastula to early gastrula stage. We describe a method for aspirating cleavage products from the blastocoele cavity for immunoblot analysis. Genetics Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders Camila F. Almeida1, Emma C. Frair1, Nianyuan Huang1, Reid Neinast2, Kim L. McBride2,3,4,6, Robert B. Weiss5, Kevin M. Flanigan1,6, Nicolas Wein1,6 1Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies. Developmental Biology Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy Srishti Kotiyal*1, Alexis Fulbright*1, Liam K. O'Brien*1, Kimberley J. Evason1 1Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute, University of Utah School of Medicine Here we demonstrate a method for quantifying liver size in larval zebrafish, providing a way to assess the effects of genetic and pharmacologic manipulations on liver growth and development. Cancer Research Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response Mattie J. Casey*1, Katarzyna Modzelewska*1, Daniela Anderson1, James Goodman1, Elena F. Boer1, Laura Jimenez1, Douglas Grossman1,2, Rodney A. Stewart1 1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses. Biology Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique Srividya Bhaskara1 1Department of Radiation Oncology, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA. Biology Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis Lingyan Xing1,2,3, Tyler S. Quist1, Tamara J. Stevenson1, Timothy J. Dahlem4, Joshua L. Bonkowsky1,2,3,5 1Division of Pediatric Neurology, Department of Pediatrics, University of Utah School of Medicine, 2Department of Neurobiology and Anatomy, University of Utah School of Medicine, 3Interdepartmental Program in Neurosciences, University of Utah School of Medicine, 4Mutation Generation and Detection Core, HSC Core Research Facility, University of Utah School of Medicine, 5Department of Neurology, University of Utah School of Medicine PCR combined with high-resolution melt analysis (HRMA) is demonstrated as a rapid and efficient method to genotype zebrafish.
