Louisiana State University Health Sciences Center-Shreveport 1 article published in JoVE Biology Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy Richa Aishwarya1, Chowdhury S. Abdullah1, Naznin Sultana Remex2, Sadia Nitu1, Brandon Hartman1, Judy King1, Md. Shenuarin Bhuiyan1,2 1Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center-Shreveport The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.
