University of Colorado-Anschutz School of Medicine 1 article published in JoVE Immunology and Infection Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry Loni Perrenoud1, James Conley1, Leslie J. Berg1 1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine Calcium influx, a measure of T-cell signaling, is an effective way to analyze responses to T-cell receptor stimulation. This protocol for multiplexing Indo-1 with panels of antibodies directed at cell surface molecules takes advantage of the highly flexible capabilities of full spectrum flow cytometry.
