Children's Health Research InstituteView Institution's Website
2 articles published in JoVE
Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro Paul William Dyce1,2 1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, The University of Western Ontario, 2Children's Health Research Institute In recent years the formation of germ cells using in vitro culture methods has been demonstrated using several different types of somatic stem cells. This article will visually present the differentiation of newborn mouse derived stem cells to early oocyte-like cells.
Single Oocyte Bisulfite Mutagenesis Michelle M. Denomme1,2,3, Liyue Zhang3, Mellissa R.W. Mann1,2,3 1Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Children's Health Research Institute Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.