2 articles published in JoVE
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection Margaret H. Magdesian1,2,3, Madeleine Anthonisen1, G. Monserratt Lopez-Ayon1, Xue Ying Chua1, Matthew Rigby1, Peter Grütter1 1Department of Physics, McGill University, 2Department of Neurology and Neurosurgery, Montreal Neurological Institute, 3Ananda Devices This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.
Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling Mame Daro Faye1, Tyson E Graber2, Martin Holcik3 1Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 2Montreal Neurological Institute, 3Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Pediatrics, University of Ottawa The ability of cells to adapt to stress is crucial for their survival. Regulation of mRNA translation is one such adaptation strategy, providing for rapid regulation of the proteome. Here, we provide a standardized polysome profiling protocol to identify specific mRNAs that are selectively translated under stress conditions.