2 articles published in JoVE
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones Louis Masclef*1, Uriarte Maxime*1, Oumaima Ahmed1, Nadine Sen Nkwe1, Haithem Barbour1, Nicholas V.G. Iannantuono1, Amina Boubekeur1, Salima Daou1,2, El Bachir Affar1,2 1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae Tali Farine1,2, Michael Parsons2, Stephen Lye1,2,3, Oksana Shynlova1,2,3 1Department of Physiology, University of Toronto, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 3Department of Obstetrics and Gynecology, University of Toronto This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.