Toronto General Hospital

5 articles published in JoVE

• Medicine

  
  Using a Chemical Biopsy for Graft Quality Assessment Iga Stryjak*¹, Natalia Warmuzińska*¹, Kamil Łuczykowski¹, Matyas Hamar², Peter Urbanellis², Emilia Wojtal³, Marek Masztalerz³, Markus Selzner^2,4, Zbigniew Włodarczyk³, Barbara Bojko^{1 1}Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, ²Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, ³Department of Transplantology and General Surgery, Collegium Medicum in Bydgoszcz, Antoni Jurasz University Hospital No. 1 in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland, ⁴Department of Medicine, Toronto General Hospital The protocol presents utilization of the chemical biopsy approach followed by comprehensive metabolomic and lipidomic analysis for quality assessment of kidney grafts allocated for transplantation.

• Medicine

  
  Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol J. Moritz Kaths^1,2, Juan Echeverri^1,3, Nicolas Goldaracena¹, Kristine S. Louis¹, Paul Yip⁴, Rohan John⁴, Istvan Mucsi⁵, Anand Ghanekar¹, Darius Bagli⁶, Markus Selzner¹, Lisa A. Robinson^{2 1}Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, ²Division of Nephrology, The Hospital for Sick Children, ³Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, ⁴Laboratory Medicine and Pathobiology, Toronto General Hospital, ⁵Department of Medicine, Toronto General Hospital, ⁶Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.

• Medicine

  
  Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation J. Moritz Kaths^1,2, Vinzent N. Spetzler¹, Nicolas Goldaracena¹, Juan Echeverri¹, Kristine S. Louis¹, Daniel B. Foltys³, Mari Strempel⁴, Paul Yip⁵, Rohan John⁵, Istvan Mucsi¹, Anand Ghanekar¹, Darius Bagli^6,7, Lisa Robinson², Markus Selzner^{1 1}Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, ²Division of Nephrology, The Hospital for Sick Children, Toronto, ³Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, ⁴Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, ⁵Laboratory Medicine & Pathobiology, Toronto General Hospital, ⁶Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, ⁷Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to  transplantation.

• Medicine

  
  Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt Vinzent N. Spetzler¹, Nicolas Goldaracena¹, Jan M. Knaak¹, Kristine S. Louis¹, Nazia Selzner¹, Markus Selzner^{1 1}Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital Experimental animal research plays a pivotal role in the development of clinical transplantation practice. The porcine orthotopic liver transplantation model (OLTx) closely resembles human conditions and is frequently used in clinically oriented research. The following protocol contains all information for a reliable porcine OLTx model using an active porto-caval-jugular shunt.

• Medicine

  
  Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts Jan M. Knaak*^1,2, Vinzent N. Spetzler*^1,2, Nicolas Goldaracena^1,2, Kristine S. Louis^1,2, Nazia Selzner^1,3, Markus Selzner^{1,2 1}Multi Organ Transplant Program, Toronto General Hospital, ²Department of Surgery, Toronto General Hospital, ³Department of Medicine, Toronto General Hospital Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.