Universite Laval 7 articles published in JoVE Biology Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods Coralie Goetz*1, Nissa Niboucha*1, Eric Jubinville1, Julie Jean1 1Département des Sciences des Aliments, Institut sur la Nutrition et les Aliments Fonctionnels, Université Laval This protocol describes an approach combining static and dynamic methods to evaluate the efficacy of organic peroxyacids for eradicating biofilms in the dairy industry. This approach may also be used to test the effectiveness of new biological or chemical formulations for controlling biofilms. Biology Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice Michael Shum1, Zhiqiang Zhou2, Marc Liesa2,3,4 1Department of Molecular Medicine, Faculty of Medicine, Universite Laval, 2Department of Medicine, Division of Endocrinology, David Geffen School of Medicine at UCLA, 3Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 4Molecular Biology Institute at UCLA This manuscript describes a protocol to measure the basal metabolic rate and the oxidative capacity of thermogenic adipocytes in obese mice. Immunology and Infection Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain Fernando González Ibanez1,2, Katherine Picard1,2, Maude Bordeleau1,3, Kaushik Sharma1,2,4, Kanchan Bisht1,2,4, Marie-Ève Tremblay1,2 1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, 3Integrated Program in Neuroscience, McGill University, 4Center for Brain Immunology and Glia (BIG), University of Virginia This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue. Neuroscience Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals Ekaterina Martianova1, Sage Aronson2,3, Christophe D. Proulx1 1CERVO Brain Research Center, Department of Psychiatry and Neurosciences, Université Laval, 2Center for Neural Circuits and Behavior, Department of Neuroscience and Section of Neurobiology, Division of Biology, University of California at San Diego, 3Neurophotometrics Ltd. This protocol details how to implement and perform multi-fiber photometry recordings, how to correct for calcium-independent artifacts, and important considerations for dual-color photometry imaging. Developmental Biology Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform Reza Salehi*1, Stephen C.M. Tsoi*1, Marcos G. Colazo2, Divakar J. Ambrose1,2, Claude Robert3, Michael K. Dyck1 1Department of Agricultural, Food and Nutritional Science, University of Alberta, 2Livestock Research Branch, Alberta Agriculture and Forestry, 3Laboratory of Functional Genomics of Early Embryonic Development, Université Laval Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA. Engineering Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements Michel Olivier1,2, Marc-Daniel Gagnon1, Joé Habel1 1Centre d'optique, photonique et laser, Université Laval, 2Département de physique, Cégep Garneau A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions. Biology RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling Audrey Chabrat*1,2, Hélène Doucet-Beaupré*1,2, Martin Lévesque1,2 1Department of Psychiatry and Neurosciences, Faculty of Medicine, Université Laval, 2Centre de recherche de l'Institut universitaire en santé mentale de Québec Gene expression analysis of a subset of cells in a specific tissue represents a major challenge. This article describes how to isolate high-quality total RNA from a specific cell population by combining a rapid immunolabeling method with laser capture microdissection.
