2 articles published in JoVE
Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model Lee Chin1,2, Elina Korpela3, Anthony Kim1, Darren Yohan2, Carolyn Niu4, Brian C. Wilson3, Stanley K. Liu1,3 1Department of Radiation Oncology, University of Toronto, 2Department of Physics, Ryerson University, 3Department of Medical Biophysics, University of Toronto, 4Ontario Cancer Institute / Campbell Family Institute for Cancer Research We present a diffuse optical spectroscopic (DOS) approach that provides quantitative optical biomarkers of skin response to radiation. We describe DOS instrumentation design, optical parameters extraction algorithms and the animal handling procedures required to yield representative data from a pre-clinical mouse model of radiation induced erythema.
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation Cheuk Y. Ho*1, Christopher H. Choy*1, Roberto J. Botelho1 1Department of Chemistry and Biology, Program in Molecular Science, Ryerson University Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with 3H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.