Ciudad Universitaria, Coyoacan 1 article published in JoVE Biology Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean Kalpana Nanjareddy*1, Manoj-Kumar Arthikala*1, Alma-Leticia Aguirre1, Brenda-Mariana Gómez1, Miguel Lara2 1Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM), 2Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Coyoacan Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
