Universidad Autonoma de Queretaro 2 articles published in JoVE Immunology and Infection Quantitation of Rabies Virus in Various Bovine Brain Structures Edith Rojas-Anaya1, Daniela Anaya-Razo2, Isabel Bárcenas-Reyes3, Elizabeth Loza-Rubio1 1Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, 2Campus Toluca, Universidad del Valle de México, 3Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription. Biology Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism Ivanna Karina Olivares-Marin1, Juan Carlos González-Hernández2, Carlos Regalado-Gonzalez1, Luis Alberto Madrigal-Perez3 1Department of Chemistry, Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering, Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.
