2 articles published in JoVE
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment Candace D. Blancett1, Mitchell K. Monninger1, Chrystal A. Nguessan1, Kathleen A. Kuehl1, Cynthia A. Rossi2, Scott P. Olschner2, Priscilla L. Williams2, Steven L. Goodman3, Mei G. Sun1 1Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.
Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines Emily D. Cisney1, Stefan Fernandez1, Shannan I. Hall1, Gale A. Krietz1, Robert G. Ulrich1 1U.S. Army Medical Research Institute of Infectious Diseases Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.