2 articles published in JoVE
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays Karla R Sanchez1, Mahlet D Mersha1, Harbinder S Dhillon1, Murali K Temburni1 1Department of Biological Sciences, Delaware State University Exposure to environmental toxins can acutely impact developing embryos. Endocrine disrupting chemicals such as bisphenols are known to adversely affect the nervous system. Here we describe a protocol using an in vitro vertebrate (chick embryo) neuronal network model to study the functional impact of toxin exposure on early embryos.
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants Mahlet D. Mersha1, Karla R. Sanchez2, Murali K. Temburni2, Harbinder S. Dhillon2 1Department of Neurology, Johns Hopkins School of Medicine, 2Department of Biological Sciences, Delaware State University Exposure to environmental toxicants can acutely impact development with long-term effects. Detailed protocols are provided to illustrate a strategy using an effective lab model to study the effect of early embryonic exposure to bisphenol A. We provide fecundity and behavioral assays to monitor the effectiveness of our toxicant exposure bio-assays.