Allen Institute for Brain Science 3 articles published in JoVE Neuroscience Injections of AAV Vectors for Optogenetics in Anesthetized and Awake Behaving Non-Human Primate Brain Yoshiko Kojima1,2, Jonathan T. Ting2,3,4, Robijanto Soetedjo2,4, Shane D. Gibson2,4, Gregory D. Horwitz2,4 1Dept. of Otolaryngology - Head and Neck Surgery, University of Washington, 2Washington National Primate Research Center, University of Washington, 3Allen Institute for Brain Science, 4Dept. of Physiology & Biophysics, University of Washington As currently implemented, optogenetics in non-human primates requires injection of viral vectors into the brain. An optimal injection method should be reliable and, for many applications, capable of targeting individual sites of arbitrary depth that are readily and unambiguously identified in postmortem histology. An injection method with these properties is presented. Neuroscience Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method Jonathan T. Ting*1, Brian R. Lee*1, Peter Chong1, Gilberto Soler-Llavina1, Charles Cobbs2, Christof Koch1, Hongkui Zeng1, Ed Lein1 1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications. Neuroscience Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy Ludovico Silvestri1, Alessandro Bria2,3, Irene Costantini1, Leonardo Sacconi1,4, Hanchuan Peng5, Giulio Iannello2, Francesco Saverio Pavone1,4,6,7 1European Laboratory for Non-linear Spectroscopy (LENS), 2Integrated Research Centre, University Campus Bio-medico of Rome, 3DAEMI, University of Cassino, 4National Institute of Optics (CNR-INO), 5Allen Institute for Brain Science, 6Department of Physics, University of Florence, 7ICON Foundation, Sesto Fiorentino, Italy In this article we describe the full experimental procedure to reconstruct, with high resolution, the fine brain anatomy of fluorescently labeled mouse brains. The described protocol includes sample preparation and clearing, specimen mounting for imaging, data post-processing and multi-scale visualization.
