2 articles published in JoVE
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin Despina Soteriou1, Lana Kostic1, Egor Sedov1, Yahav Yosefzon1, Hermann Steller2, Yaron Fuchs1 1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
Imaging Subcellular Structures in the Living Zebrafish Embryo Peter Engerer1, Gabriela Plucinska1,2, Rachel Thong1,3, Laura Trovò1, Dominik Paquet4,5,6, Leanne Godinho1 1Institute of Neuronal Cell Biology, Technische Universität München, 2Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3Faculty of Biology, Ludwig-Maximilians-Universität-München, 4Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians-Universität-München, 5German Center for Neurodegenerative Diseases, 6Laboratory of Brain Development and Repair, The Rockefeller University Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.