2 articles published in JoVE
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots Thomas B. Jacobs1, Gregory B. Martin1,2 1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University Using DNA assembly, multiple CRISPR vectors can be constructed in parallel in a single cloning reaction, making the construction of large numbers of CRISPR vectors a simple task. Tomato hairy roots are an excellent model system to validate CRISPR vectors and generate mutant materials.
Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses Hui Jiang1, Hugues Barbier2, Thomas Brutnell1 1Donald Danforth Plant Science Center, 2Boyce Thompson Institute We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.