University of Arizona College of Medicine 7 articles published in JoVE Medicine Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum Patrick G. Hughes1, Kate E. Hughes2, Mary J. Hughes3,4, Lindsay Weaver4, Lauren E. Falvo4, Anna M. Bona4, Dylan Cooper4, Cherri Hobgood4, Rami A. Ahmed4 1Florida Atlantic University Schmidt College of Medicine, 2Department of Emergency Medicine, University of Arizona College of Medicine, 3Department of Osteopathic Medical Specialties, College of Osteopathic Medicine, Michigan State University, 4Indiana University School of Medicine The goal is to demonstrate how to apply the rapid cycle deliberate practice debriefing technique to the GRIEV_ING death notification curriculum. Medicine Setup and Execution Of the Blindfolded Code Training Exercise Patrick G. Hughes1, Kate E. Hughes2, Rami A. Ahmed3 1Florida Atlantic University Schmidt College of Medicine, 2Department of Emergency Medicine, University of Arizona College of Medicine, 3Department of Emergency Medicine, Indiana University School of Medicine The blindfolded code training exercise, which blindfolds the team leader in a code resuscitation simulation, is an advanced teaching technique to improve closed-loop communication, organizational skills, and critical thinking. Biology Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse Zhen Wang1,4, Ramon Ocadiz-Ruiz1, Sinju Sundaresan1, Lin Ding1, Michael Hayes1, Nirakar Sahoo3, Haoxing Xu1,2, Juanita L. Merchant1,2,5 1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis. Medicine Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection Hao Chen1, Robert Klein1, Stacy Arnold1, Yiying Wang2, Setsuko Chambers3,4, Wenxin Zheng5,6 1Department of Pathology, University of Arizona College of Medicine, 2 We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens. Medicine Exergaming in Older People Living with HIV Improves Balance, Mobility and Ameliorates Some Aspects of Frailty Suhitha Veeravelli1, Bijan Najafi3, Ivan Marin3, Fernando Blumenkron2, Shannon Smith2, Stephen A. Klotz2 1Department of Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP), College of Medicine, University of Arizona, 2Department of Medicine, Division of Infectious Disease, College of Medicine, University of Arizona, 3Interdisciplinary Consortium on Advanced Motion Performance (iCAMP), Division of Vascular Surgery and Endovascular Therapy, Michael E. DeBakey Department of Surgery, Baylor College of Medicine Persons infected with HIV are often frail, depressed and live a sedentary lifestyle for which conventional exercise is too taxing. Here, we present an exercise protocol that ameliorates aspects of frailty in HIV-infected persons. An exergame integrating cognitive control was developed using biosensors that measured balance, weight-shifting and obstacle crossing. Biology Analysis of LINE-1 Retrotransposition at the Single Nucleus Level Pasano Bojang1, Kenneth S. Ramos1,2 1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Arizona College of Medicine, 2Center for Applied Genetics and Genomic Medicine, University of Arizona College of Medicine Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1. Medicine The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis Rishu Gupta1,2, Maya Debbaneh2,3, Daniel Butler2,4, Monica Huynh2,5, Ethan Levin2, Argentina Leon2, John Koo2, Wilson Liao2 1Keck School of Medicine, University of Southern California, 2Psoriasis and Skin Treatment Center Dermatology, University of California, San Francisco, 3University of California Irvine School of Medicine, 4University of Arizona College of Medicine, 5Chicago College of Osteopathic Medicine Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.