Phoenix Children's Hospital 1 article published in JoVE Neuroscience Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons Megan N. Evilsizor1,2, Helen F. Ray-Jones1,3, Jonathan Lifshitz1,2,4,5, Jenna Ziebell1,2 1Department of Child Health, University of Arizona College of Medicine - Phoenix, 2 This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.