3 articles published in JoVE
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections Yuu Ohno1, Riley Murphy2, Matthew Choi3, Weijun Ou4, Rachita K. Sumbria4,5 1Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences, Chapman University, 3Keck Science Department, Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, 5Department of Neurology, University of California, Irvine The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain Alzahra J. Al Omran1, Hannah C. Saternos1, Tongyu Liu2, Surya M. Nauli3, Wissam A. AbouAlaiwi1 1Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute, University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties.
Quantifying Agonist Activity at G Protein-coupled Receptors Frederick J. Ehlert1, Hinako Suga2, Michael T. Griffin3 1Department of Pharmacology, University of California, Irvine, 2Department of Pharmacology, University of California, 3Schmid College of Science, Chapman University A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.