PBS Biotech, Inc. 2 articles published in JoVE Bioengineering Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining Teresa P. Silva1,2, Tiago G. Fernandes1, Diogo E. S. Nogueira1, Carlos A. V. Rodrigues1, Evguenia P. Bekman1,2,3, Yas Hashimura4, Sunghoon Jung4, Brian Lee4, Maria Carmo-Fonseca2, Joaquim M. S. Cabral1 1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA This protocol describes a dynamic culture system to produce controlled size aggregates of human pluripotent stem cells and further stimulate differentiation in cerebellar organoids under chemically-defined and feeder-free conditions using a single-use bioreactor. Bioengineering Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System Kristina M. Obom1, Patrick J. Cummings1, Janelle A. Ciafardoni1, Yasunori Hashimura2, Daniel Giroux2 1Center for Biotechnology Education, Johns Hopkins University, 2PBS Biotech, Inc. Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.
