UCSF Benioff Children's Hospital 2 articles published in JoVE Immunology and Infection Isolation of Primary Mouse Lung Endothelial Cells Erika Wong1,2, Nina Nguyen2, Judith Hellman2 1 In this article, primary lung endothelial cells were isolated and cultured from neonatal mice. Immunology and Infection Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface Gabrielle A. Rizzuto1,2, Mirhan Kapidzic3, Matthew Gormley3, Anna I. Bakardjiev1,4,5 1 A simple method to establish primary human placental (villous) and decidual organ cultures is described. Villous and decidual organ cultures are invaluable tools for studying pathogenesis at the human maternal-fetal interface. Infection with the facultative intracellular bacterium Listeria monocytogenes is demonstrated.