1 article published in JoVE
Anti-Nuclear Antibody Screening Using HEp-2 Cells Carol Buchner1, Cassandra Bryant2, Anna Eslami3, Gabriella Lakos4 1IFA Development Manager, INOVA Diagnostics, Inc., 2IFA Development, INOVA Diagnostics, Inc., 3Product Manager, Rheumatology, INOVA Diagnostics, Inc., 4Medical Director, INOVA Diagnostics, Inc. Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.