Sanford Consortium for Regenerative Medicine 2 articles published in JoVE Developmental Biology Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease Sandra L. Leibel1,2,3, Rachael N. McVicar2,3, Alicia M. Winquist2,3, Evan Y. Snyder1,2,3 1Department of Pediatrics, University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme. Neuroscience Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation Barbara Calabrese1,2, Regina M. Powers1,2, Alexandria J. Slepian1,2, Shelley Halpain1,2 1Division of Biological Sciences, University of California, San Diego, 2Sanford Consortium for Regenerative Medicine This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.
